| 摘要 |
PROBLEM TO BE SOLVED: To provide methods of imaging tissues or cells, the method having desirable in vivo properties that result in improved signal-to-background ratio.SOLUTION: The invention relates to methods of imaging tissues or cells, the methods including (a) bring the tissues or cells into contact with an imaging agent comprising a dye or conjugate thereof, the conjugate comprising a target ligand attached to the dye, in which the dye or conjugate has a net charge of +1, 0, or -1 and comprises one or more ionic groups; (b) irradiating the tissues or cells at a wavelength absorbed by the dye or conjugate; and (c) detecting an optical signal from the irradiated tissues or cells, in which the signal-to-background ratio of the detected optical signal is at least about 1.1, thereby imaging the tissue or cells. Preferably, the dye is cyanine based dyes in Fig.7. Preferably, the target ligand of the imaging agent selectively binds to a prostate specific membrane antigen.SELECTED DRAWING: Figure 7 |
