ANTIBODY LIKE PROTEIN

摘要

A general method and recombinant nucleic acid sequences, by means which the method selects a recombinant protein containing an FHA domain for binding a target molecule from a library proteins with a high-throughput method of creating protein variations within the FHA domain in non-conserved or non-structural sequences of the FHA scaffold, and the library may also be in the form of a phagemid or phage library wherein the ALP nucleic acid sequence is inserted into a vector capable of allowing the vector and expressed ALP protein from being virally packaged, and the recombinant nucleic acid sequences which are randomly mutated at varying non-conserved or non-structural FHA domain sequences.

主权项

1. A library of isolated nucleic acids that encodes for a library of variant proteins where each of the variant proteins comprises:
a) an FHA domain b) each of the variant proteins differ from each other by partial random alterations in the amino acid sequence of FHA domain wherein the random alterations are not substantially disruptive to the structural scaffold of the FHA domain; c) the random alterations originating from random alterations of the isolated nucleic acids an originating nucleic acid sequence that encodes for an originating protein comprising the wild-type FHA domain; d) the randomly altered nucleotide sequences are adjacent to each other with a minimum length of two nucleotides and that the randomly altered nucleotide sequences encode for amino acids that are not disruptive to the structural scaffold of the FHA domain.