发明名称 |
METHODS AND KITS FOR DETECTING PROTEIN-PROTEIN INTERACTIONS |
摘要 |
The present invention relates to methods and kits for detection protein-protein interactions. In particular, the present invention relates to a method for detecting the binding between a first polypeptide (A) and a second polypeptide (B) in a cell comprising i) providing a cell that expresses (a) a polypeptide (GFP1-9) comprising an amino acid sequence having at least 90% of identity with the amino acid sequence selected from the group consisting of SEQ ID NO: 1-4 (b) a first fusion protein wherein the polypeptide (A) is fused to a polypeptide (GFP10) having an amino an amino acid sequence having at least 90% of identity with the amino acid sequence selected from the group consisting of SEQ ID NO:5-7 (c) a second fusion protein wherein the polypeptide (B) is fused to a polypeptide (GFP11) having an amino an amino acid sequence having at least 90% of identity with the amino acid sequence selected from the group consisting of SEQ ID NO: 8-9 and (d) an intrabody specific for the complex formed by the self-assembly of the first, second and third polypeptides (a), (b) and (c) ii) detecting the fluorescence wherein when the fluorescence is detected it is concluded that the polypeptide (A) binds to polypeptide (B) and wherein the fluorescence is not detected it is concluded that the polypeptides (A) does not bind to polypeptide (B). |
申请公布号 |
WO2016207313(A1) |
申请公布日期 |
2016.12.29 |
申请号 |
WO2016EP64612 |
申请日期 |
2016.06.23 |
申请人 |
INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE);UNIVERSITÉ PAUL SABATIER TOULOUSE III |
发明人 |
CABANTOUS, Stéphanie;KORAÏCHI, Faten;FAVRE, Gilles |
分类号 |
G01N33/542;G01N33/68 |
主分类号 |
G01N33/542 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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