MODIFIED MICROORGANISMS FOR CHEMICAL PRODUCTION

摘要

The present invention relates to increasing xylose utilization in industrial microbe by inducing mutations in the regulator genes, crp and xylR. Thus the invention is directed to isolated nucleic acid sequences that encode mutations in the crp gene and the xylR gene and recombinant bacterium that express mutated CRP and XylR. In some embodiments, the mutation results in a point mutation at residue 142 of the CRP protein and/or at point mutation at residues 121, 182 and/or 363 of the XylR protein (based on the protein sequences in E. coli). The invention also includes methods of using the recombinant bacterium.

主权项

1. A recombinant bacterium expressing a CRP protein having an amino acid sequence set forth in SEQ ID NO:11, wherein X1 is selected from the group consisting of: D, P, H, and conservative substitutions thereof.