PROCEDURE FOR THE RAPID DETERMINATION OF BACTERIAL SUSCEPTIBILITY TO ANTIBIOTICS THAT INHIBIT PROTEIN SYNTHESIS

摘要

The present invention relates to am method for the rapid evaluation of bacterial susceptibility or non-susceptibility of bacteria to antibiotics that inhibit protein synthesis. The rationale is to identify bacterial responses that depend or are influenced by protein synthesis. If this response is prevented or reduced by the antibiotic that inhibits the protein synthesis, the bacteria are susceptible to this antibiotic. Otherwise, if the response keeps similar despite the incubation with the antibiotic, the bacteria are no susceptible or resistant to this antibiotic. These responses could be determined at the DNA level, cell wall level, morphological level or any other experimental approach, including metabolic, bio-chemical, physiological or genetic processes

主权项

1. A method for rapidly evaluating the susceptibility of bacteria sample to a protein synthesis inhibiting antibiotic comprising:
incubating a first portion of the bacteria sample with a protein synthesis inhibiting antibiotic; adding an agent to the first portion of the bacteria sample, the agent being selected to induce a bacterial response which depends on or is influenced by protein synthesis; incubating a second portion of the bacteria sample with the agent selected to induce a bacterial response which depends on or is influenced by protein synthesis; evaluating the bacterial response in the first portion and the second portion of the bacteria sample; and classifying the bacteria sample as susceptible or non-susceptible to the protein synthesis inhibiting antibiotic based on the evaluation of the first and second portions of the bacteria sample.