| 摘要 |
<p>AA method of determining the presence or absence of a target microorganism in a test sample, the method comprising: flagging a bacteriophage (70) with a detectable flag; combining flagged bacteriophage with the sample and providing conditions sufficient to allow flagged bacteriophage to attach to the microorganism creating a bacteriophage exposed sample; performing a target separation process, the process capable of separating the bacteriophage exposed sample into a target microorganism portion containing target microorganisms present in the sample and a portion containing flagged bacteriophage not bound to the target bacteria; and assaying at least one of the portions to detect the presence or absence of the flag to determine the presence or absence of target microorganisms in the sample. The flag (90) can be colloidal gold particles, lectins, aptomers, immunoadhesion molecules, biotin molecules, latex beads, fluorophores or other color-based molecule, and the like. The invention also provides a lateral flow strip (140) having a conjugate pad (143) containing flagged bacteriophage (142) and an immobilization zone (146) containing capture elements (170) specific to the target bacteria.</p> |
