Methods And Compositions For Incorporating Nucleotides

摘要

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

主权项

1. A composition comprising a mixture of labeled and unlabeled reversible terminators in solution, said terminators comprising nucleotide analogues of dCTP, dGTP and dATP, wherein the concentration of the unlabeled nucleotide analogue of dCTP is present in said solution at a higher concentration than the labeled nucleotide analogue of dCTP, wherein the concentration of the unlabeled nucleotide analogue of dGTP is present in said solution at a higher concentration than the labeled nucleotide analogue of dGTP, wherein the concentration of the unlabeled nucleotide analogue of dATP is present in said solution at a higher concentration than the labeled nucleotide analogue of dATP, and wherein each labeled and unlabeled nucleotide analogue contains a removable chemical moiety capping the 3′-OH group.