MULTI-FOCAL STRUCTURED ILLUMINATION MICROSCOPY SYSTEMS AND METHODS

摘要

A multi-focal selective illumination microscopy (SIM) system for generating multi-focal patterns of a sample is disclosed. The multi-focal SIM system performs a focusing, scaling and summing operation on each multi-focal pattern in a sequence of multi-focal patterns that completely scan the sample to produce a high resolution composite image.

主权项

1. A microscopy system comprising:
a light source for transmitting a single light beam; a beam splitter for splitting the single light beam into a plurality of light beams forming a multi-focal pattern; a scanner for scanning the plurality of light beams that forms the multi-focal pattern onto a sample such that the sample generates a plurality of fluorescent emissions with each multi-focal pattern; a focusing component defining an aperture configured to block out-of-focus fluorescence emissions of the plurality of fluorescent emissions for each multi-focal pattern and allowing through in-focus fluorescent emissions to pass through the aperture; a scaling component for scaling down the plurality of in-focus fluorescent emissions for each multi-focal pattern such that each of the plurality of in-focus fluorescent emissions is scaled down to produce a plurality of scaled in-focus fluorescent emissions for each multi-focal pattern; and a summing component for summing each of the plurality of scaled in-focus fluorescent emissions to produce a plurality of summed, scaled in-focus fluorescent emissions that form a composite image of the plurality of summed, scaled in-focus fluorescent emissions.