Factor VII polypeptides that are modified and uses thereof

摘要

Modified factor VII polypeptides and uses thereof are provided. Such modified FVII polypeptides include Factor VIIa and other forms of Factor VII. Among modified FVII polypeptides provided are those that have altered activities, typically altered procoagulant activity, including increased procoagulant activities. Hence, such modified polypeptides are therapeutics.

主权项

1. A modified factor VII (FVII) polypeptide, comprising amino acid replacements at positions corresponding to positions 286 and 298 in a FVII polypeptide having the sequence of amino acids set forth in SEQ ID NO:3, wherein:
the amino acid replacement at position 286 is Arg (R), and at position 298 is Gln (Q); the modified FVII polypeptide, when in an activated form, exhibits procoagulant activity; and the amino acid sequence of the modified FVII polypeptide has at least 90% sequence identity with a polypeptide having the sequence of amino acids set forth in any of SEQ ID NOs:1-3.