METHODS AND DEVICES FOR HIGH THROUGHPUT SCREENING OF CONDITIONS AFFECTING STEM CELL DIFFERENTIATION

摘要

Disclosed are methods and systems for testing the effects of various morphogens and/or feeder cells on the differentiation of pluripotent cells. The assays described herein can be used for determining the optimum conditions that lead to differentiation of stem cells. Once the optimum conditions for stem cell differentiation are determined, such cells may be used in a variety of therapies.

主权项

1. A method for identifying a pluripotency profile of stem cells comprising:
culturing one or more stem cells in a cell culturing system, wherein the cell culturing system comprises a cell culturing plate comprising a layer of cell culturing substrate, a cell culture site comprising the one or more stem cells, and one or more morphogen sites comprising one or more morphogens, wherein the one or more morphogen sites are each positioned peripheral to the cell culture site; incubating the cell culturing system under conditions suitable to allow the one or more stem cells to differentiate into one or more differentiated cells and migrate on the cell culturing plate in response to the one or more morphogens; and determining the migration pattern of the one or more differentiated cells on the cell culturing plate, wherein the migration pattern of the one or more differentiated cells is indicative of the pluripotency profile of the population of stem cells.