| 摘要 |
<p>The present invention provides a simple, rapid and reliable method for sequencing nucleic acid inserts directly from a plasmid DNA which comprises the steps of: spinning down an overnight cell culture to obtain a pellet; resuspending the pellet; heating the resuspended pellet; and separating the insoluble cell debris from the DNA-containing supernatant, whereby the DNA-containing fraction is useful as template DNA for automated sequencing.</p> |
