PROCESS FOR ISOLATING AND PURIFYING NUCLEOTIDE-ACTIVATED SUGARS FROM BIOLOGICAL SOURCES

摘要

The present invention relates to an improved process for isolating and purifying sugar nucleotides, in particular cytidine monophosphate-activated N-acetylneuraminic acid (CMP-Nana), from biological sources. The sugar nucleotides can be obtained from a sugar nucleotide-containing solution, which has been freed of proteins by alcoholic precipitation, by means of an improved column-chromato-graphic method which uses silica gel as the stationary phase, at a purity which is so great that a subsequent desalting step is all that is required to isolate them.