| 摘要 |
<p>A method of separating an upper aqueous phase from a lower organic phase in a two phase system. The method comprises the following steps: a) adding wax to the two phase system, b) heating the two phase system from a), whereby the wax is melted in the organic phase, c) cooling the two phase system from b) to solidify the wax-organic phase, d) withdrawing the upper aqueous phase. Further, the invention comprises a method of isolating RNA from cells by phenol-chloroform extraction in which an upper aqueous phase and a lower phenol-chloroform phase is formed, the aqueous phase containing the RNA. The method comprises the following steps: a) adding a solid way to the extraction mixture of cells, phenol and chloroform, b) heating the mixture to melt the wax, whereby the wax is mixed in the phenol-chloroform phase, c) cooling the mixture to solidify the wax-phenol-chloroform phase, d) withdrawing the upper aqueous phase containing the RNA.</p> |
