| 摘要 |
The present invention provides a method of producing L-glutamic acid by fermentation, comprising the steps of culturing a mutant of an L-glutamic acid-producing microorganism of the genus Brevibacterium or Corynebacterium which has lower alpha -ketoglutaric acid dehydrogenase activity compared with the wild strains from which said mutant is derived, in a liquid nutrient culture medium containing biotin at a concentration of 10 to 1000 mu g/l without adding a biotin activity-suppressing substance thereto; producing and accumulating L-glutamic acid in the culture solution; and recovering L-glutamic acid from said culture solution. According to the method of the present invention, it is possible to industrially produce L-glutamic acid by fermentation in a more economical and efficient manner. |
