| 摘要 |
<p>A thermostable creatine amidinohydrolase having the following physicochemical properties: (a) hydrolyzing 1 mol of creatine to give 1 mol of sarcosine and 1 mol of urea; (b) having a substrate specificity to creatine; (c) having an optimum pH range of 7.0 to 8.0; (d) having a stable pH range of 4.0 to 11.0; (e) having the optimum function temperature of around 45 °C; (f) being stable at 53 °C; and (g) having a molecular weight of 92,000 Da (determined by gel filtration method).</p> |
