GENE DELIVERY SYSTEM CONTAINING DESIRED FOREIGN GENE IN VIRUS-LIKE PARTICLES

摘要

PURPOSE: A gene delivery system containing a desired foreign gene in virus-like particles is provided, thereby the desired foreign gene can be stably delivered into the cell without decomposing by nucleases and the desired foreign gene also stable in vivo because the virus-like particles do not have nucleic acid derived from virus. CONSTITUTION: The gene delivery system contains a desired foreign gene in virus-like particles consisting of only viral structure proteins, wherein the viral structure protein is a capside protein L1 derived from human papillomavirus(HPV); the foreign gene is associated with immunity; and the foreign gene associated with immunity is selected from the group consisting of cytokine gene, chemokine gene, interleukin-2 (IL-2) gene, tumor suppressor gene H19, cell cycle participating gene p21 and angiogenesis inhibitor flk-1. The method for producing the gene delivery system containing the desired foreign gene comprises the steps of: treating the virus-like particles with an appropriate reducing agent to be unit capsomer proteins; adding the desired foreign gene into the unit capsomer protein and allowing them to react; and removing the reducing agent to refold the unit capsomer protein.