VERFAHREN ZUR HERSTELLUNG VON PEPTIDEN

摘要

A process for production of a peptide (target peptide) comprising: A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the following formula: A-L-B wherein, B is a target peptide, A is a protective peptide comprised of 90-200 amino acid residues, and L is a linker peptide positioned between the C-terminal of said protective peptide and the N-terminal of said target peptide and selected so that said fusion protein is treated by an enzyme or chemical substance, the above-mentioned target peptide is separated, and said protective peptide and linker peptide are selected so that the isoelectric point of the entire fusion protein A-L-B is within a range of 4.9-6.9; B) obtaining an insoluble fraction comprising inclusion bodies by homogenization of the cultured cells of said transformant body; C) solubilizing of a fusion protein in said inclusion bodies by treatment of said insoluble fraction with solubilizing agent; and, D) cleaving of the peptide bond between the C-terminal of the linker amino acid residue and the N-terminal of the target peptide of said solubilized fusion protein to release said target peptide from the other peptides followed by purification of said target peptide. The present invention is able to express a target peptide in a large amount and accumulate said target peptide in host cells in the form of inclusion bodies.