NEW FLAVIN REDUCTASE AND DNA ENCODING THE ENZYME AND TRANSFORMED CELL TRANSFORMED WITH THE DNA

摘要

PROBLEM TO BE SOLVED: To obtain a new flavin reductase having high thermal stability, and a DNA encoding the same.

SOLUTION: The flavin reductase uses NADH as electron donor and FMN (flavin mononucleotide) as electron acceptor as substrates, respectively, exhibits an action for producing NAD+ (nicotinamide adeniue dinuclcotide +) and FMNF2, has the optimal temperature of the reaction in a range of 65 to 75°C, and holds ≥80% of the activity even when left at pH 7 at a temperature of ≤70°C for 30 min.

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