发明名称 |
Combinatorial libraries of proteins having the scaffold structure of C-type lectin-like domains |
摘要 |
A family of protein libraries comprising CTLDS (C-type Lectin-Like Domains) in which internal polypeptide loop-regions lining the ligand binding sites in CTLDs have been replaced with ensembles of completely or partially randomised polypeptide segments. Tetranectin CTLDs were chosen as framework for the preferred embodiment; and versatile phagemid vectors useful in the generation and manipulation of human and murine tetranectin CTLD libraries are disclosed. Tetranectin CTLDs in monomeric as well as in trimeric form are efficiently displayed as gene III fusions in fully functional form by the recombinant fd phage display vector. CTLD derivatives with affinity for new ligands may readily be isolated from libraries of vectors displaying CTLDs, in which loop-regions have been randomised, using one or more rounds of enrichment by screening or selection followed by amplification of the enriched subpopulation in each round. The efficiency with which protein products containing CTLDs with new binding properties can be produced, e.g. by bacterial expression in in vitro refolding, in mono-, tri-, or multimeric formats provides important advantages in terms of simplicity, cost and efficiency of generation, production and diagnostic or therapeutic applications in comparison to recombinant antibody derivatives.
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申请公布号 |
NZ526312(A) |
申请公布日期 |
2004.11.26 |
申请号 |
NZ20010526312 |
申请日期 |
2001.12.13 |
申请人 |
BOREAN PHARMA A/S |
发明人 |
ETZERODT, MICHAEL;HOLTET, THOR LAS;GRAVERSEN, NIELS JONAS HEILSKOV;THOGERSEN, HANS CHRISTIAN |
分类号 |
C12N15/09;C07K14/47;C07K19/00;C12N15/10;C12N15/12;C12P21/02;(IPC1-7):C07K14/47;C07K4/12;C12N5/04;C12N5/06;C12N7/01;C12N5/10;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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