| 摘要 |
<P>PROBLEM TO BE SOLVED: To obtain a plasmid vector which enables the efficient and simple expression of a foreign protein in Escherichia coli. <P>SOLUTION: The plasmid vector containing a promoter sequence capable of functioning in Escherichia coli cell, an SD (liposome binding) sequence, cloning sites, a medicine-resistant gene, and a replication origin capable of functioning in the Escherichia coli cell is characterized by follows. The cloning sites exist at two positions near to the 5'-side and 3'-side of the SD sequence. The first and second cloning sites contain restriction enzyme-recognizing sequences, respectively, which produce cohesive ends, when cleaved with a restriction enzyme. At least one of the combinations of the restriction enzyme-recognizing sequences contained in the first and second cloning sites is a combination of the restriction enzyme-recognizing sequences which have common cohesive ends and are respectively produced, when cleaved with the restriction enzyme. <P>COPYRIGHT: (C)2005,JPO&NCIPI |
