METHODS AND KITS FOR DETECTING NUCLEIC ACID SEQUENCES OF INTEREST USING DNA-BINDING PROTEIN DOMAIN

摘要

The present invention relates to a method for the detection of a specific nucleic acid. Specifically, the invention provides a method and kits for detecting the presence of a specific nucleic acid using engineered DNA-binding domains such as Transcription Activator Like-Effector (TALE) domain or modular base-per-base binding domains (MBBBD). The method of the invention is particularly useful for in vitro diagnostic application.

主权项

1. A method of detecting a nucleic acid sequence of interest comprising:
(a) Providing nucleic acids which can comprise the nucleic acid sequence of interest; (b) Providing at least one DNA binding domain capable of binding said nucleic acid sequence of interest; (c) Contacting said nucleic acids with said DNA binding domain; (d) Removing unbound said DNA binding domain and nucleic acids and; (e) Detecting the presence or the absence of the nucleic acid sequence of interest.