发明名称 |
Methods for producing mature protein in vertebrate host cells. |
摘要 |
<p>An expression vector capable of expressing in a vertebrate host cell culture a desired protein and a secondary protein, which vector comprises a DNA sequence encoding for a desired protein and a DNA sequence encoding for a secondary protein wherein both said DNA sequences are operably linked to the same promoter sequence and separated by translational stop and start codons.
<??>The secondary sequence provides for a convenient screening marker, both for transformants in general, and for transformants showing high expression levels for the primary sequence, as well as serving as a control device whereby the expression of a desired polypeptide can be regulated, most frequently enhanced. </p> |
申请公布号 |
EP0117058(A2) |
申请公布日期 |
1984.08.29 |
申请号 |
EP19840300298 |
申请日期 |
1984.01.18 |
申请人 |
GENENTECH, INC. |
发明人 |
LEVINSON, ARTHUR DAVID;SIMONSEN, CHRISTIAN CLINTON |
分类号 |
C12N5/10;C07H21/04;C07K14/00;C07K14/02;C12N5/00;C12N15/00;C12N15/09;C12N15/67;C12N15/85;C12P21/00;C12Q1/04;C12R1/91;(IPC1-7):12N15/00;12N5/00;12P21/02 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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