发明名称 |
Method for discriminating surface stained lymphocytes |
摘要 |
A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.
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申请公布号 |
US5122453(A) |
申请公布日期 |
1992.06.16 |
申请号 |
US19880188803 |
申请日期 |
1988.05.03 |
申请人 |
TECHNICON INSTRUMENTS CORPORATION |
发明人 |
MARTIN, GRACE E.;KANTER, ROBERT J.;KIM, YOUNG R.;ORNSTEIN, LEONARD;ANSLEY, HUDSON R. |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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