| 摘要 |
Antigens, immunogens, inocula, antibodies, and particularly diagnostic methods and systems relating to Epstein-Barr virus nuclear antigen (EBNA) are disclosed. The diagnostic methods and systems utilize a synthetic, random copolymer polypeptide containing about 8 to about 40 amino acid residues that includes the overlapping five and six amino acid residue sequences -Gly-R1-Gly-R2-Gly-(i) wherein R1 and R2 are amino acid residues selected from Ala, Asn, Arg, Gly, Leu, Pro, Ser, and Thr with the provision that R1 and R2 are not both Gly; and -Gly-Ala-Gly-Gly-Ala-Gly-. (ii) The polypeptide contains at least 50 mole percent Gly residues. The diagnostic method and system are particularly useful for assaying for the stage of mononucleois disease, and the presence of nasopharynegeal carcinoma. Assaying for anti-Epstein-Barr virus nuclear antigen antibodies is carried out by mixing a body sample with the synthetic polypeptide, allowing antibodies in the sample to immunoreact with the polypeptide to form an immunoreactant and determining the immunoreactant. In another embodiment, the synthetic polypeptide is fixed to a solid phase and a solid phase immunoreactant obtained from immunoreacting with antibodies in the sample is mixed with anti-heavy chain antibodies to form a second immunoreactant which is determined. The anti-antibodies can be anti-IgG or anti-IgM, and the anti-antibodies can be linked to an indicating means such as an enzyme.
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