METHODS AND SYSTEMS FOR VOLUME VARIATION MODELING IN DIGITAL PCR

摘要

A method for performing digital polymerase chain reaction (dPCR) is provided. The method comprises partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions. At least one partition includes at least one target nucleic acid. The method further includes determining a model for volume variation of the plurality of partitions and determining a fraction of partitions including at least one target nucleic acid. The method includes generating a concentration of target nucleic acids in the biological sample based on the model for volume variation and the fraction of partitions including at least one target nucleic acid.