| 摘要 |
<p>A method of assaying for non-familial Alzheimer's disease in a live patient, by preparing washed platelets from the blood of the patient; inserting a pH probe in the platelets in the form of a fluorescent dye such as 2',7'-bis (carboxyethyl)- 5(6)-carboxyfluorescein; inhibiting alkalinization of platelet response by blocking Na+/H+ transfer across the platelet membranes with dimethyl amiloride; stimulating the platelets with α-thrombin so that the cytoplasm of the stimulated platelets shows a characteristic acidification response or change in cytoplasmic pH; measuring the cytoplasmic pH of the platelets following stimulation; and comparing the measured pH with the corresponding stimulated cytoplasmic pH in platelets from normal individuals.</p> |
