| 摘要 |
PURPOSE:To provide an apparatus for culturing a cell of a living body capable of embodying the high-density culture of the cell of the living body for a long period using a porous microcarrier. CONSTITUTION:This apparatus for culturing a cell of a living body is provided by installing a screen (3a) in a culture tank 1, preventing a microcarrier from leaking out of a culture solution, dissolving oxygen in the culture solution in a tank 2 for submerged aeration by the submerged aeration, circulating the resultant oxygen-enriched culture solution, efficiently supplying oxygen to the cell, simultaneously monitoring the turbidity and dissolved oxygen concentration (DO) in the culture solution, judging the concentration distribution of the microcarrier in the culture tank and oxygen consumption by the cell in the culture tank, changing the internal pressure in the tank 2 for the submerged aeration, oxygen concentration and aeration gas flow rate of the aeration gas based on the resultant information, balancing the sedimenting rate of the microcarrier with the circulation rate of the culture solution, supplying the oxygen required for the cell and further reducing the vibrating operation with a vibrator 11 and the existing concentration of the microcarrier near the screen (3a).
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