发明名称 |
COMPOSITIONS FOR ENZYME CATALYZED TEMPLATE-INDEPENDENT CREATION OF PHOSPHODIESTER BONDS USING PROTECTED NUCLEOTIDES |
摘要 |
<p>A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.</p> |
申请公布号 |
EP0781293(A4) |
申请公布日期 |
1999.05.26 |
申请号 |
EP19950932399 |
申请日期 |
1995.08.31 |
申请人 |
HIATT, ANDREW C. ROSE-HIATT BIOTECHNOLOGY PARTNERSHIP;ROSE, FLOYD |
发明人 |
HIATT, ANDREW C.;ROSE, FLOYD |
分类号 |
C12N15/09;C07H19/10;C07H19/20;C07H21/00;C12P19/30;C12P19/34;(IPC1-7):C07H21/00;C07H21/04;C07H21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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