| 摘要 |
PURPOSE: A process for producing glutathione(GSH) is provided, thereby providing an economic way for producing GSH, allowing to improve the productivity of the GSH producing yeasts as well as the rapid isolation of GSH overproducing mutants, the inexpensive cultivation of such mutants, the increase the GSH content of the biomass and the rapid and effective extraction of GSH from the biomass. CONSTITUTION: The process for producing glutathione comprises the steps of: (a) the obtainment of a biomass pre-culture by pre-cultivating, in aerobic conditions, a strain of a yeast genus selected from Saccharomyces, Hansenula, Pichia, Candida, Cryptococcus, Schizosaccharomyces, Sporobolomyces, Bullera, Bulleromyces, Cryptococcus, Filobasidiella, Lipomyces, Rhodotorula or a stable mutant thereof, wherein the glutathione content per biomass unit is higher than 1.2% w/w; (b) the cultivation, in aerobic conditions, of the resulting biomass pre-culture such that the density of the resulting cultured biomass is higher than 50 g/l; (c) the activation of the cultured biomass; and (d) the recovery of glutathione from the cultured biomass resulting from step (c) by extracting glutathione, at a pH equal to or lower than 6, and purifying the resulting glutathione.
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