Methods and devices for detecting structural changes in a molecule measuring electrochemical impedance

摘要

The disclosure relates to a method of detecting a structural change in a molecule that is attached to a surface that is electrically conductive, wherein the method includes monitoring the phase of the electrochemical impedance at the surface, and wherein a change in the phase indicates a change in the structure of the molecule. The disclosure also relates to arrays and methods for making arrays having molecules, such as polypeptides, attached to electrically conductive surfaces, such as electrodes.

主权项

1. A method of detecting binding of an entity to a molecule, said molecule being attached to a surface, said surface being electrically conductive, wherein the method comprises
monitoring the phase of the electrochemical impedance at said surface over a range of frequencies; and detecting change in the monitored phase of the electrochemical impedance at said surface over said range of frequencies, wherein said change in the monitored phase is dependent on the concentration of said entity and said change indicates said binding to said molecule.