PCR reaction cleanup buffers

摘要

The present disclosure relates to buffers containing polyols for use with affinity-binding and/or magnetically susceptible thermoplastic particles and methods of making and use thereof.

主权项

1. A buffer for use with a magnetic affinity-binding thermoplastic particle extraction process comprising at least one soluble polyol having a base structure of Formula 3: wherein R3 is OH, CH3, (—OCH2CH2O)nH, or wherein R1, R2, R4, R5, R6 and R7 are each independently H, or (—CH2CH2O)nH; wherein each n is independently an integer of one or greater; wherein at least one of R1, R2, R4, R5, R6 and R7 is (—CH2CH2O)nH; wherein the total number of (—CH2CH2O)nH groups is Nt; and, wherein Nt is at least 6.