发明名称 |
BACTERIAL BETA-GALACTOSIDASE, IMMOBILIZED FORMS THEREOF, PROCESS FOR THEIR PREPARATION AND USE IN THE HYDROLYSIS OF LACTOSE, AND NOVEL BACTERIA |
摘要 |
A novel beta -galactosidase comprises an enzyme having a thermal stability such that when in purified form it has an activity half life of at least 11/2 hours at 55 DEG C, at least 1 hour at 60 DEG C and at least 10 minutes at 65 DEG C, as measured using ONPG as substrate, said enzyme being derived from a bacterium of species Bacillus stearothermophilus. There is also provided a process for the production of the novel beta -galactosidase, comprising culturing in a medium containing appropriate nutrients a micro-organism of species B. stearothermophilus having characteristics similar to or the same as the strains identified as NCIB 11407-11413, and which contains the beta -galactosidase of the invention. Additionally there is provided a process for the hydrolysis of lactose to a product comprising glucose and galactose which comprises contacting lactose with the beta -galactosidase of the invention e.g. at a temperature of at least 55 DEG C preferably at least 60 DEG C or especially about 65 DEG C. |
申请公布号 |
DE2963289(D1) |
申请公布日期 |
1982.08.26 |
申请号 |
DE19792963289 |
申请日期 |
1979.06.07 |
申请人 |
NATIONAL RESEARCH DEVELOPMENT CORPORATION |
发明人 |
GRIFFITHS, MANSELL WILLIAM;MUIR, DAVID DONALD;PHILLIPS, JOHN DAVID |
分类号 |
C12N9/38;C12N11/04;C12N11/10;C12P19/14;(IPC1-7):C12N9/38;C07G7/00;C07H3/02 |
主分类号 |
C12N9/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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