| 摘要 |
Prodn. of high-purity factor (VIII) by . of fibrinogen, globulins and albumins from cryoprecipitate is effected by (a) suspending the thawed cryoprecipitate in a 2-fold vol. of H2O contg. 1l3 U/ml of Na heparin at 10-20 deg. C, (b) adjusting the pH to 7-8 with dil. HOAc, (c) stirring at 10-20 deg. C while slowly adding an equal vol. of 7-30% aq. EtOH contg. 1-3 U/mol of Na heparin, (d) adjusting the pH to 6.8-7.2 with dil. HOAc, (e) slowly cooling to 10-15 deg. C while stirring, (f) sepig. the ppte., and (g) working up the supernatant in known manner. Step (g) is effected by adding A-(OH)3, centrifuging, filtering the supernatant, adding Na cholate/TNBP to inactivate viruses removing the Na cholate/TNBP by oil extraction, and ultrafiltering the aq. phase.
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