Morpholino-mediated increase in soluble Flt-1 expression results in decreased ocular and tumor neovascularization

摘要

Methods of inhibiting lymphangiogenesis and/or angiogenesis in a subject are provided. In one aspect, for example, a method of inhibiting angiogenesis in a subject can include binding an antisense morpholino to an mRNA splicing site of VEGFR1 selected from exon13_intron13 junction, intron13_exon14 junction, or a combination thereof. In another aspect, the morpholino includes a member selected from VEGFR1_MOe13, VEGFR1_MOi13, or a combination thereof.

主权项

1. A method of inhibiting angiogenesis in a subject wherein the method comprises binding an antisense morpholino to a splicing site of VEGR1 mRNA in the subject wherein the splicing site is selected from the group consisting of exon13_intron13 junction, intron 13_exon14 junction, or a combination thereof such that the VEGFR1 mRNA is spliced into an sFlt-1 isoform, wherein the antisense morpholino is at least 75% identical to SEQ ID NO: 1 or is at least 75% identical to SEQ ID NO: 2.