LUMINESCENT SUBSTRATE FOR USE IN ARTIFICIAL BIOLUMINESCENT ENZYME

摘要

The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.

主权项

1. Use of a compound as a luminescent substrate for a polypeptide comprising an amino acid sequence (i) or (ii) below, and having a copepod luciferase activity:
(i) the amino acid sequence of SEQ ID No: 1; and (ii) the amino acid sequence of SEQ ID No: 1 in which one or more amino acids are deleted in at least one of a region corresponding to positions 1-31 and a region corresponding to positions 217-221, wherein the compound is represented by the following formula (1) wherein Xa represents a phenyl group optionally substituted with halogen or a naphthyl group, Xb represents phenyl, and Mc represents hydrogen, hydroxy, or thiol.