发明名称 |
Plant genes involved in nitrate uptake and metabolism |
摘要 |
The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants. |
申请公布号 |
US9523099(B2) |
申请公布日期 |
2016.12.20 |
申请号 |
US201414462017 |
申请日期 |
2014.08.18 |
申请人 |
Iowa State University Research Foundation, Inc. |
发明人 |
Schnable Patrick S.;Dash Sudhansu |
分类号 |
A01H5/00;A01H1/00;C12N15/00;C12N15/82;C07K14/415;C07H21/04 |
主分类号 |
A01H5/00 |
代理机构 |
LeClairRyan, a Professional Corporation |
代理人 |
LeClairRyan, a Professional Corporation |
主权项 |
1. An expression vector comprising a nucleic acid construct comprising:
a nucleic acid molecule that is up-regulated by nitrogen in corn; a 5′ DNA promoter sequence, wherein the DNA promoter sequence is a nitrogen inducible plant promoter; and and a 3′ terminator sequence, wherein the nucleic acid molecule, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleic acid molecule, wherein the DNA promoter sequence is heterologous to the nucleic acid molecule, and wherein the nucleic acid molecule either: (a) encodes a polypeptide having the amino acid sequence of SEQ ID NO:45; or (b) comprises the nucleotide sequence of SEQ ID NO:44; or (c) comprises the coding portion of the nucleotide sequence of SEQ ID NO:44. |
地址 |
Ames IA US |