Generation of Patient-Specific Differentiated Cell Types by Epigenetic Induction

摘要

Disclosure of a mammalian cytoplasmic donor cell line. Disclosure of a patient specific cell line. Methods to obtain a mammalian cytoplasmic donor cell line by fusing a differentiated mammalian cell and a functionally enucleated mammalian embryonic cell line. Methods to obtain a mammalian cytoplasmic donor cell line by fusing a differentiated mammalian cell and a functionally enucleated human cancer cell. Methods to obtain a patient specific cell line of a cell type similar to a mammalian cytoplasmic donor cell line by functionally enucleating the mammalian cytoplasmic donor cell line and fusing the functionally enucleated mammalian cytoplasmic donor cell line with a differentiated cell obtained from the patient. A method of treatment of a human patient by administering the patient-specific cell line to the patient.

主权项

1. A method of preparing a self-propagating dopaminergic neuron cell specific to a human patient, the method comprising:
(a) obtaining a human cancer cell; (b) treating the human cancer cell with a DNA interchelating agent to obtain a DNA interchelating agent treated human cancer cell, (c) treating the DNA interchelating agent treated human cancer cell with an agglutinin to obtain an agglutinin-treated human cancer cell; (d) fusing the agglutinin-treated human cancer cell from step (c) with a human dopaminergic neuron cell to obtain a self-propagating human dopaminergic neuron cytoplasmic donor cell; (e) treating the self-propagating human dopaminergic neuron cytoplasmic donor cell from step (d) with a DNA interchelating agent to obtain a DNA interchelating agent treated human dopaminergic neuron cytoplasmic donor cell; (f) treating the DNA interchelating agent treated human dopaminergic neuron cytoplasmic donor cell from step (e) with an agglutinin to obtain an agglutinin-treated human dopaminergic neuron cytoplasmic donor cell; (g) fusing the agglutinin-treated human dopaminergic neuron cytoplasmic donor cell from step (f) with a histone deacetylase inhibitor treated somatic cell from a human patient whereby a self-propagating dopaminergic neuron cell specific to the human patient is obtained.