主权项 |
1. A vaccine against at least one antigen from a bovine pathogen, said vaccine comprising a bovine herpes virus-1 (BoHV-1) genome from a low virulence BoHV-1 having genetic material encoding the at least one antigen which is heterologous to BoHV-1 inserted between two converging BoHV-1 genes wherein the insertion does not substantially down-regulate expression of the BoHV-1 genes, wherein the genetic material encoding the at least one antigen is inserted between the polyadenylation signals of two converging genes at a functionally equivalent site in another BoHV-1 to a site selected from the group consisting of between 16600 to 16700, 22400 to 22500; 40,700 to 40,800; 58,000 to 59,000; 67,000 to 68,000; 74,000 to 76,000; 84,000 to 85,000; 90,000 to 91,000; and 96,000 to 97,000 of BoHV-1 reference sequence GenBank Accession No. AJ004801. |