Postpartum cells derived from umbilical cord tissue, and methods of making and using the same

摘要

Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.

主权项

1. A cell culture comprising an isolated cell obtained from mammalian umbilical cord tissue substantially free of blood cultured in a culture medium, wherein said isolated cell is capable of self-renewal and expansion of its numbers in culture, expresses CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, HLA-A, HLA-B, and HLA-C, does not express CD31, CD34, CD45, CD117, CD141, HLA-DR, HLA-DP and HLA-DQ, has increased expression, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, of a gene for interleukin 8, reticulon 1 and chemokine (C-X-C motif) ligand 3, and produces granulocyte chemotactic protein-2 (GCP-2), and wherein the culture medium is chemically-defined medium or medium with added mammalian serum.