| 摘要 |
The invention disclosed herein comprises a laboratory electrophoresis apparatus which overcomes many drawbacks of the previously known apparatus by combining a number of operations in the same unit, thereby eliminating the need for moving a gel between analytic jobs. The apparatus of the invention has a gel casting platform permanently mounted in a buffer container with retractable dams on two opposite sides to retain the gel casting liquid prior to solidification. The gel remains in place on the platform and the dams are retracted for filling the buffer container and a vertical electric field is applied to compact the sample placed in the sample wells to the bottom. The electrophoresis in a horizontal direction is performed and may be observed by means of an included UV radiation source capable of causing the ethidium bromide treated molecular samples to emit visible light, which is observable through a UV opaque safety cover. A viewing hood including a UV cross-linking source may be substituted for viewing, especially during blotting or eluting operations, for which the gel is not moved from its casting platform. To do blotting, either a complete transfer membrane or a partial, single band membrane with a novel holder is used. A further operation is performed by the replacement of the viewing hood with a photography hood for the placement of a camera, capable of either UV or visible light photography. By this combination of features, the multi-purpose apparatus of the invention permits all necessary molecular analytic operations without the movement of the gel, thus improving the efficiency and reliability of results.
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