| 摘要 |
Two cell systems are disclosed for the production, detection and isolation of Human Neurological Disease Virus (HNDV) from patients with neurological disease. HNDV is maintained and reproduced in two interleukin-2 independent malignant lymphocyte cell lines, CM-1 and CM-2. Compositions are derived from the novel viral isolate, including the whole virus, proteins, polypeptides, polynucleotide sequences and antibodies to antigenic sites on the virus. These compositions are useful in a variety of techniques for the diagnosis and treatment of human encephalomyelitits such as multiple sclerosis. In addition, these compositions are useful in a variety of techniques for the detection of and vaccination against HNDV. Detection methods include immunoassays for both the virus and antibodies to the virus, and the use of polynucleotide probes to detect the viral genome. Vaccines include both wholly and partially inactivated viruses and subunit vaccines. |
