| 摘要 |
Sample fluids including biological particles are mixed with a stain solution, and stained biological particles are fed into a flow through cell. Still frame images of the particles flowing through the flow-through cell are photographed by a television camera, and are processed for particle classification. Prior to such processing of biological particle images, a calibration coefficient is calculated using reference particles. The reference particles may include polystyrene resin which contains functional radicals such as sulfonic radicals, and are stained by mixing the suspended fluid containing these reference particles with the stain solution. The calibration coefficient is calculated according to the information on the stain in the still frame images, and is used to calibrate biological particle data.
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