METHOD OF PREPARING AN ATTENUATED STRAIN OF BACTERIUM SALMONELLA

摘要

FIELD: microbiology. SUBSTANCE: method provides preparing strains of bacterium Salmonella containing nonreversible mutations in genes determining the formation of aromatic compound metabolites. Mutations concern the genes aro A, aro C or aro E and they are inserted by transposon mutagenesis or transformation using plasmid DNA involving inactivated gene aro. These strains can be used for vaccine preparing at high effectiveness. EFFECT: improved method of preparing. 5 cl, 1 dwg , 3 tblp