发明名称 |
Recombinant DNA and expression vector for the repressible and inducible expression of foreign genes |
摘要 |
The recombinant DNA according to the present invention contains a regulator region which is different from the pfl gene; a promoter region upstream from the gene which contains a -35/-10 promoter sequence; a regulator region upstream from the promoter region which contains a sequence (I) 5'-GAGATATGATCTATATCAATTTC-3' or a 23 base pair sequence which is at least 80% identical to positions 6-10 and 15-19 of said sequence (I) but not having C at position 8 and/or G at position 17. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule and methods are also described for expression the DNA.
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申请公布号 |
US5830720(A) |
申请公布日期 |
1998.11.03 |
申请号 |
US19950386198 |
申请日期 |
1995.02.09 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
BOECK, AUGUST;SAWERS, ROBERT GARY;JARSCH, MICHAEL;HERBST, ROLAND |
分类号 |
C12N1/21;C12N15/70;(IPC1-7):C12N15/63;C07H21/04;C12P21/02 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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