发明名称 |
Preparation of stable formulations of lipid-nucleic acid complexes for efficient in vivo delivery |
摘要 |
The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab' fragment attached to a hydrophilic polymer.
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申请公布号 |
US2002182249(A1) |
申请公布日期 |
2002.12.05 |
申请号 |
US20020121962 |
申请日期 |
2002.04.12 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
PAPAHADJOPOULOS DEMETRIOS;HONG KEELUNG;ZHENG WEIWEN |
分类号 |
A61K9/00;A61K9/127;A61K31/711;A61K47/10;A61K47/48;A61K48/00;C07H21/00;(IPC1-7):A61K48/00;C12N15/88 |
主分类号 |
A61K9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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