PLANT DF2SSP PROMOTER ISOLATED FROM DOUGLAS-FIR 25 SEED STORAGE PROTEIN GENE CAPABLE OF DRIVING EXPRESSION.

摘要

A promoter (termed df2SSP and designated herein SEQ ID NO: 17) for a seed-storage protein from Douglas fir (Pseudotsuga menziesii) capable of driving the expression of a transgene as well as the endogenous seed-storage protein gene is described. It is also contemplated that promoters such as the CaMV 35S promoter may be altered through the introduction of sequences found in the df2SSP promoter. Figure 2 is a schematic depiction of the df2SSP promoter. The promoter elements are identified as follows: I denotes E-box motifs; II denotes RY-repeated elements; III denotes AT-rich regions; IV denotes ACGT-core regions; V denotes opaque-2-like elements; VI denotes gymnosperm-like sequences. The connecting the elements together represents the nucleic acid strand. The regions of the line between the elements represent inter-element regions. The nucleic acid sequence encoding the Douglas fir 2S seed-storage protein (gPm2SI; SEQ ID NO: 10) is shown below. gaattcgtac tctgctttgt atcgagttat tgtgttt ttg ttttacacgt ggcagggatc 60 ttgtttgtta tttctatgca cttagtcact actgtaattt ggttaagaat tttaattcag 120 atgcagttaa agatgaagag catatctagt tgaaaaacta tgagcttaac tagcttcgga 180 ggcagagcta aagtagttag gacagaagta aaaacatcat aaatagaaat agagcatgta 240 gctttgttag atctaacatc aaaaccataa acaacagggt tagtttacct tatttctcaa 300 gtgcaaaggc aggaacatat ccataggttc caaatgcata gtttggagca gtggttccag 360 atttcgacaa agcatatcta catcctatag attaagttga actggcaaca taagtgacag 420 catatctaca tctggtaggc tatggcatca acggtggtgt gtcttatcaa gggtcatcca 480 ccttgtgaaa gggtgtgaca tgcattaaca aagtcctacc atcaaattgt gaaatgcatt 540 atgcatcaga taggtggcac catcatgttt caagtaagta agatagtgtt gtgaagtggt 600 gattccctca taccagacta gaaaaattaa tatttaatgt taatattaat gttagcatta 660 ttgaacttca taatctctta gtgaactaag tgggttatgt ctgggttatt tcttgagatt 720 acgtcgttcc caatagaagt gttgttgcgt taaaccttgc cgatgaaaac aacgtgaaac 780 atctacacca ccttacgaaa aatggcatta cgagaagagg aaggcagttt atcatcacct 840 aattagtttg tctaaatgca ataagcctaa gtagtgttgt tgagacgcta gactg cactg 900 tcgcggattc cacatgcccg caagattcct ctaatcgaaa gacgcggcga ttggaaatag 960 aggtaggctt tctttctcag cgtggaatcc gaattggggc atgctttaca tgtgttcgtt 1020 tcgtcatcgt ctgtattcta cgtccctaca gcatacagac accttcaatt cccgggcttt 1080 tataaatacc cacttactct tcccaacttc a 1111.