Benchtop fluorescence molecular beacons detector and reader

摘要

A low cost benchtop molecular beacons (oligonucleotide probes) reader utilizes an array of light emitting diodes to excite the fluorescence of the reaction of the combining of the molecular beacons with actual DNA strands. If the Molecular beacons match with the DNA the reaction will fluoresce a known wavelengths. If there is no match, the reaction will not fluoresce. The molecular beacons reader uses an array of light emitting diodes at given wavelengths. The unit also incorporates the proper filters depending on the wavelength required. The unit has interchangeable cartridges with the filters and light emitting diodes that can be easily changed. The system is used after the PCR process (polymerase chain reaction) and the samples are viewed in PCR strips or PCR plates directly out of a thermocycler. This unit serves as a low cost go-no-go test with easy to use equipment.