| 摘要 |
The invention relates to a portable device and to a method for cryoinclusion of a tissue material for microscopic examination, used especially in hospitals and in research laboratories. According to the invention, the device consists of a vat (A) which, together with a case cover (B), determines an interior space for a freezing chamber which is thermally insulated with an insulating layer (3) inside the vat (A) and with an upper layer (7) in the case cover (B), in the vat (A) there being mounted a metal block (1) with increased specific heat and thermal conductivity, regularly made of aluminium, provided on one side with tapered recesses (2) having the role of shaping cavities for inclusion of tissues into the ice mass. As claimed by the invention, the method consists in introducing and keeping the device without the case cover, in a freezer of ultra-low temperature, until reaching the metal block temperature of -80°C, mounting the case cover and some object-holder pieces when opening the freezer and before getting the remaining assembly out of the -80°C medium, transporting the device, in normal conditions, to the sampling spot, then, when the operation of inclusion is needed, the cover is detached, the tissue fragment is placed inside the inclusion vat in the shaping cavities, then the freezing medium is poured over it, after which the object-holder pieces and the case-type cover are placed thereon, the freezing medium placed above the block plane getting into the object-holder piece grooves and facilitating the binding of samples with tissue material, after freezing, by rotating the object-holder piece rod, the ice block with the embedded tissue material is detached and, in the end, the object-holder piece with uni-directional grooves allows the detachment of the tissue material by pressing the ice block, with the embedded tissue material, this way, the tissue material may be used immediately or stored for further use. |
