| 摘要 |
An immunological process for the detection and determination of a substance using an immunologically active reaction partner which is marked with an enzyme and an immunologically active reaction partner which is or will be bound to a water-insoluble carrier comprises incubation of the substance to be determined with the immunologically active reaction partners, subsequent separation of solid and liquid phases and measurement of the extent of the enzyme marking either in the solid or in the liquid phase as a measure of the amount of the substance to be determined, the substance to be determined and the immunologically active reaction partners all being present in the reaction mixture at the start of the single incubation step used for the immunological reaction. The substance to be determined may be an antigen, and the immunologically active reaction partners may be antibodies from different clones, which are directed towards different epitopes of the antigen. |
