| 摘要 |
<p>Insect pheromone long chain aldehydes are assayed in liquid samples, down to extremely small quantities, by a bioluminescent technique. A sample of reduced flavine mononucleotide and bacterial luciferase enzyme is treated with hydroxylamine, to scavenge out background aldehyde, and is then reacted with the aldehyde containing liquid sample for assay, in the presence of water and oxygen, to cause oxidation of the reduced flavine mononucleotide, with emission of light. By determination of the maximum light intensity and optionally the decay curve of the emitted light, the amounts of aldehyde in the liquid sample can be accurately assayed, and information can be obtained concerning the chemical identity of the aldehyde. The process is useful in the monitoring of atmospheric aldehyde concentrations, in association with insect pheromone traps and matins disruption by pheromones.</p> |
